1Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana-125004, India
2Department of Gynaecology & Obstetrics, College of Veterinary and Animal Science, Orissa University of Agriculture and Technology, Bhubaneswar-751 003, Odisha, India
3Department of Veterinary Microbiology, Arawali Veterinary College Affiliated to Rajasthan University of Veterinary and Animal Sciences, Bikaner, N.H.-52 Jaipur Road, V.P.O. Bajor, Sikar-332 001, Rajasthan, India
Department of Veterinary Parasitology, Faculty of Veterinary Science and Animal Husbandry, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, India
*Corresponding author's e-mail: ganguly38@gmail.com
Online published on 15 March, 2019.
Bovine cryptosporidiosisis primarily associated with neonatal diarrhoea with higher morbidity than mortality in young calves till they attain immunological maturity. The present investigation relates to a report on the shedding of Cryptosporidium oocyst intwo buffalocalvesof buffalo farm, Lala LajpatRai University of Veterinary and Animal Sciences, Hisarfrom 1st days up to 3 month of age at 15 days interval using simple conventional microscopy. By using formol-ether concentration technique followed by modified Ziehl-Neelsen (ZN) acid fast staining, Cryptosporidium oocysts were concentrated and identified. The Cryptosporidium oocysts appeared as reddish pink coloured bodies against a bluish/greenish coloured background at oil immersion using ZN staining kit. The maximum oocyst shedding was observed (2.3 oocyst/field) during 16 to 30 days of age. The oocyst shedding gradually decreased with increase in age afterwards clearly indicating the disease of young buffalo calves.
Buffalo calves, Conventional microscopy, Cryptosporidium spp, Oocyst shedding
