Central University Laboratory, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai-600 051, Tamil Nadu, India
*Corresponding author's e-mail: maranvet@yahoo.com
Online published on 26 February, 2019.
Respiratory Disease (CRD) among chickens of various age groups. The present study describes the isolation, molecular detection and histopathological changes associated with CRD in chickens. A total of 790 samples viz., trachea, lungs and air sacs were collected from chickens showing the symptoms of CRD from different parts of Tamil Nadu state. All the samples were processed for isolation and molecular detection of MG. A total of 91 samples were found positive by isolation and 105 samples were found positive through MG specific PCR targeting 16S rRNA gene. The histopathological changes in tissue samples of trachea, sinuses, air sacs and lungs collected from naturally infected M. gallisepticum infection were suggestive of subacute to chronic nature of infection. Though isolation is considered to be a gold standard, still PCR is a rapid, sensitive and cheap method for early diagnosis of MG which can help poultry farmers to avoid severe economic loss.
CRD, Histopathology, Mycoplasma gallisepticum, PCR
