1College of Animal Science and Veterinary Medicine, Heilongjiang BAYI Agricultural University, Daqing, Heilongjiang, P. R. China, 163319
College of Animal Science and Veterinary Medicine, Heilongjiang BAYI Agricultural University, Daqing, Heilongjiang, P.R. China, 163319
*Corresponding author's e-mail: lsj4396@163.com
Online published on 26 February, 2019.
LOC427400 is a novel bird gene located on chromosome Z of the chicken genome. It was deciphered in our previous study by RNA sequencing of different parts of the chicken oviduct. Here we confirmed that LOC427400 expressed significantly high in the magnum comparing with the ovary, isthmus, uterus, liver, jejunum and breast muscle (P<0.05) by quantitative real-time PCR in 5 White Leghorn layers at the age of 34 wk. The full length of transcript sequence of LOC427400 was also obtained by 3’ and 5’ RACE. The new transcript was 2831 bp and the corresponding translated protein was 343 aa. The predicted protein show 2 conserved domains, RICIN and OST-beta, which indicated that LOC427400 protein might have functions like carbohydrate-binding and signal transduction. This study illustrated that LOC47400 should be an important candidate gene related with the process of egg albumen formation and albumen biological functions.
Chicken, Egg albumen, Gene expression, LOC427400 gene, Rapid amplification of cDNA ends
