1National Bureau of Animal Genetics and Resources(NBAGR), Karnal-132001 (India)
2Animal Biotechnology Centre, National Dairy Research Institute Karnal-132001(India)
ICAR-National Dairy Research Institute, Karnal-132 001, India
*Corresponding author's e-mail: tanmayhazra08@gmail.com;
Online published on 4 November, 2017.
Due to higher market demand of buffalo milk paneer, lower price cow milk is often adulterated with higher cost buffalo milk for preparation of paneer. Till date no rapid technique is available in market to ensure that paneer is made from buffalo milk. Currently a PCR based method has been developed to authenticate the buffalo milk paneer. DNA was isolated from paneer by DNeasy Mericon food kit. A set of bovine specific primers (P1) targeting D-loop (displacement loop) of mt-DNA was selected and standardized to amplify cow DNA resulted 126bp amplicon. Using this PCR based approach even upto 1% level of cow milk adulteration in buffalo milk paneer could be detected.
Food kit, Mitochondrial gene, Paneer, PCR, Species Specific primer, Universal primer
