1Department of Biotechnology, Gauhati University, Guwahati 781 014, Assam.
2Project Directorate on Animal Disease Monitoring and Surveillance, Hebbal, Bangalore 560 024.
ICAR-National Research Centre on Pig, Rani, Guwahati-781 014, Assam, India.
Online published on 4 May, 2017.
Growth hormone releasing hormone (GHRH) plays a central role in growth and production through its influence on important metabolic activities in mammals which prompted its wide commercial use including use as a candidate marker. In present experiment, polymorphism at exon 2 and 3 of Growth Hormone Releasing Hormone gene (GHRH) was studied using PCR-RFLP in crossbred and exotic pigs, commonly found in north east India. Restriction enzyme digestion of 467 bp PCR amplicon with AluI revealed 1 genotype. Comparison of obtained sequences revealed large scale nucleotide and residue substitutions. Observed polymorphism may beassociated with widevariabilityin growth (rate) ofdifferent breeds of pig.
GHRH, PCR-RFLP, Pig, Polymorphism
