1School of Life Sciences, Shaoxing University, Shaoxing, 312000, China
2School of Life Sciences, East China Normal University, Shanghai, 200062, China
School of Life Sciences, Shaoxing University, Shaoxing, 312000, China
*Corresponding author's e-mail: luowen@usx.edu.cn
Online published on 10 February, 2017.
Transmission electron microscopy (TEM) and immunocytochemistry were used to assay the circulating hemocytes characteristics in Cherax quadricarinatus (von Martens, 1868). Three haemocyte types could be identified in circulating hemocytes by TEM, i.e. hyaline cells (HC), semigranular cells (SGC), or granular cells (GC). The circulating hemocytes from C. quadricarinatus could be separated into layers by density gradient centrifugation. The CD34+ cells which are believed to be stem cell-like cells has been discovered in the first layer via immunocytochemical assay, which has not been reported in previous studies. These CD34+ cells may be immature cells derived from a single undifferentiated precursor cell line in specialized hematopoietic tissue (HPT).
Circulating hemolytic, CD34+ cells, Density gradient centrifugation
