1Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Science, 201106, Shanghai, China
Key Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 200090, Shanghai, China
*Corresponding author's e-mail: shiming.peng@163.com; shizhh@hotmail.com
The first four authors contributed equally to this work
The effects of dietary probiotics on growth performance, innate immunity and digestive enzymes of silver pomfret were investigated. Compared to the control, diets supplemented with Bacillus subtilis, Lactobacillus plantarum and Clostridium butyricum caused significant improvements of growth performance, including final weight, weight gain, specific growth rate and feed conversion rate. A better growth performance among probiotic treatments was obtained in fish fed B. subtilis. To reveal the effects of these three probiotics on innate immunity of silver pomfret, lysozyme activity, SOD activity and IgM concentrations were examined. Results showed that silver pomfret fed diets containing C. butyricum, L. plantarum and B. subtilis had led to an enhanced innate response, notably higher lysozyme activity, SOD activity and IgM concentrations, as recorded after 30 and 60 days of feeding. In addition, study of different digestive enzymes viz lipase, protease and amylase activities showed significant higher values in fish fed the probiotic diets in comparison to the untreated group. These findings demonstrated that administration of C. butyricum, L. plantarum and B. subtilis can improve growth performance through enhanced immune response and digestive enzyme activity in silver pomfret. Furthermore, the B. subtilis is highly recommended as dietary probiotics in silver pomfret.
Digestive enzymes, Growth performance, Immune response, Probiotics, Silver pomfret
