Correlation analysis between milk and serum LPBE FMD virus type specific antibody titres in buffaloes vaccinated with polyvalent oil adjuvant FMD vaccine

The present investigation was carried out on 15 randomly selected milch buffaloes divided into three groups on the basis of lactation of an organized farm, to study the Foot and Mouth Disease Virus (FMDV) type specific antibodies in milk and serum following FMD vaccination. Milk and serum samples collected before vaccination i.e. 0 day and on 7, 14, 28, 42 and 56 days post vaccination, were analyzed for the detection of FMD virus type specific antibodies by liquid phase blocking ELISA (LPBE). Milk LPBE antibody titres against different FMD virus types were significantly different showing higher antibody titres against FMD virus type O followed by A and Asia 1. Serum LPBE antibody titres against FMD virus type O and Asia 1 were not significantly different, except for type A. Milk and serum LPBE antibody titres were positively correlated with value of regression coefficient (R) as 0.6376 and correlation was highly significant (P<0.01). Testing milk in place of sera is useful and practically advantageous in large scale epidemiological studies on milch animals after demonstration of a significant correlation between milk antibody titres and corresponding serum antibody titres in the study.