Phylogenetic analysis and comparative study of Sahiwal cattle CD14 gene

Cluster of Differentiation-14 (CD14) gene is one of the strong candidate genes for mastitis resistance trait. Bovine CD14 gene spans over 2630 bp having 2 exons. Present investigation is based on phylogenetic analysis and comparative study of CD14 gene in Sahiwal cattle. A total of 100 lactating Sahiwal cattle maintained at NDRI, Karnal (Haryana) were included in the present study. Sequence analysis revealed that Sahiwal cattle CD14 gene sequence is 73 to 99% identical with several species. Phylogenetic analysis indicated that Sahiwal cattle (Bos indicus) and Bos taurus are genetically the most similar (99%) followed by Bubalus bubalis. The predicted peptide sequence revealed 373 amino acids precursor corresponding to coding sequence of CD14 gene of Sahiwal cattle. Amino acid length of CD14 protein was found different in reported species. BLAST analysis also revealed that Sahiwal cattle CD14 protein is 73 to 100% similar with other reported species. The sites for leucine-rich repeats, leucine rich nuclear export signal, putative N-linked glycosylation, O-linked glycosylation and disulphide bridges were predicted and comparative study was done.