Expression of heat shock protein 70-1 gene in bovine peripheral blood mononuclear cells

The present study has demonstrated the expression of HSP70-1 gene in bovine peripheral blood mononuclear cells (PBMCs). Blood samples were collected aseptically by jugular vein puncture in EDTA (1mg/ml) from six apparently healthy sahiwal cows (Bos Indicus). Peripheral blood mononuclear cells (PBMCs) were separated from the blood using HiSep^TM Lymphocyte Separation Media (LSM). Total RNA was isolated from PBMCs using Trizol reagent. The purity and concentration of total RNA was checked using Nanodrop instrument whose further confirmation was made by using denaturing agarose gel (1.5%) electrophoresis. Isolated total RNA was reverse transcribed to cDNA that was used for amplification of HSP70-1 gene. Full length open reading frame (ORF) of HSP70-1 gene was amplified using specific primers pair. The presence of 1926 base pair band in denaturing agarose indicated the amplification of HSP70-1 gene. To conclude, our results demonstrate that HSP70-1 gene is expressed in bovine PBMCs.