Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2014
  • Volume: 48
  • Issue: 6

Antemortem diagnosis of rabies from skin by TaqMan real time PCR

Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 001, India

*Corresponding author's e-mail: rabiesck@gmail.com

Online published on 18 December, 2014.

Abstract

Present study revealed application of TaqMan real time PCR technique for diagnosis of rabies viral RNA from 11/20 antemortem skin samples with a sensitivity of 92.85%. The successful use of TaqMan real time PCR concluded that it was a viable, alternative tool for the antemortem diagnosis of rabies. Results correlated well with those of the post-mortem fluorescent antibody test performed on brain specimens of same animals.

Keywords

Antemortem diagnosis, Rabies, Skin, TaqMan real time PCR