Identification of genetic polymorphism in two exonic coding region of Leptin gene among indigenous and crossbred cattle

Leptin gene has its role in appetite, metabolism, growth and milk production in cattle. PCR-RFLP method was carried out to detect the polymorphism in leptin gene among Frieswal, Ongole and Sahiwal cattle. Frieswal population under study revealed that 11%, 51% and 38% are TT, CT, and CC respectively genotypes with respect to exon 2. But the same population had 22% TT, 51% CT and 27% CC genotype for first SNP in exon 3; 4% TT, 38% C Tand 58% CC genotype for second SNP in exon 3. Ongole population revealed only CT genotype with respect to exon 2, but it showed TT genotype for first SNP and CC genotype for second SNP in exon3. Sahiwal population had 4% TT, 88% CT and 8% CC genotype (exon 2), respectively. Whereas the same population revealed 4% TT, 55% CT and 41% CC genotype for first SNP in exon 3 and 4% CT and 96% CC genotype for second SNP in exon 3. Sahiwal population had no TT genotype for second SNP in exon 3.