Conservation of qinghai fine-wool sheep at somatic cell level in sterile culture

This study aimed to establish the fibroblast cell bank of Qinghai fine-wool sheep in the form of somatic cells, and to study its biological characteristics, to help for long-term conservation and sustainable use of this genetic resource. Ear marginal tissue of the Qinghai Fine-wool sheep was used to establish a fibroblast cell line by direct culture of explants and cryopreservation techniques, and the quality of the cell line was assessed. This study not only shows the effective preservation of the Qinghai Fine-wool sheep germplasm at the cellular level, but also provides a available experimental resource for fields including cell biology, genomics, post-genomics, genetic engineering and embryo engineering.