Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly -243 122, India
*Corresponding author's e-mail: drmrout@gmail.com, Preseant Address: Project Directorate on Foot and Mouth Disease, IVRI Campus, Mukteswar, Nainital, 263138, India
Online published on 3 April, 2014.
The study was made to find out the etiological agent involved in piglet diarrhoea during 20072009 in and around Bareilly. Group A rotavirus (GARV) was found as the main agent using various methods applied to intestinal and fecal samples. Out of 199 necropsied intestinal samples, none was positive in agarose gel electrophoresis, 5 (2.51%) were positive in double antibody sandwich ELISA (DAS-ELISA), 5 (2.51%) in RT-PCR and 18 (9.04%) in real-time RT PCR. Out of 96 fecal samples, 8 (8.33%) were positive in agarose gel electrophoresis, 11 (11.45%) in DAS-ELISA, 16 (16.66%) in RT-PCR and 23 (23.95%) were found positive in real-time RT PCR. Thus, it was found that real-time RT PCR, RT-PCR, ELISA and agarose gel electrophoresis have a descending order of sensitivity for detection of rotavirus as has already been reported.
Feces, Group A rotavirus, Intestine, Methods, Porcine
