CD14 gene polymorphism using HinfI restriction enzyme and its association with mastitis in Sahiwal cattle

Cluster of diffferention-14 (CD14) gene is involved in innate immunity and has been identified as one of the candidate gene for disease resistance. CD14 molecule works as cell surface receptor present on surface of inflammatory cells. Bovine CD14 gene spans over 2630 bp having 2 exons. Present study is based on identification of DNA polymorphism and to analyze the association between CD14 gene variants with incidence of mastitis in Sahiwal cattle. A total of 100 lactating Sahiwal cattle maintained at NDRI, Karnal (Haryana) were included in the present study. PCR-RFLP analysis of contig 4 using HinfI restriction enzyme showed three different genotypic patterns (CC, CD and DD). Remaining contig-enzyme combinations showed monomorphic pattern. Genotypic frequency of CC genotype was found highest (54%). Chi-square (χ²) analysis revealed that there is significant difference between these genotypes regarding mastitis incidence. CC genotyped animals were found to be less susceptible for mastitis.