Isolation and molecular characterization of bovine herpes virus-1 by polymerase chain reaction

The present study was undertaken to isolate the Bovine Herpes virus-1 (BHV-1) from cattle and buffaloes suffering from Infectious Bovine Rhinotrachitis (IBR) and confirmed by polymerase chain reaction (PCR) and compare it with the standard isolate of BHV-1. A total of 40 nasal and conjunctival swabs were collected from cattle and buffalo from different districts of Karnataka. Isolation of BHV-1 was carried out in Madin Darby Bovine Kidney (MDBK) cell lines. Out of 40 samples collected, three virus strains were isolated from three samples with one each from Mandya, Hassan, and Bangalore district. The virus isolates were confirmed and characterized by PCR using set of designed primers targeting Glycoprotein C (gC) gene of BHV-1 followed by restriction endonuclease (RE) analysis and sequencing.