Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2013
  • Volume: 47
  • Issue: 3

Detection of CD4/CD8 ratio in mice immunized with a bicistronic plasmid construct encoding a ppe gene of Mycobacterium avium paratuberculosis and a cytokine gene of murine gamma interferon*

  • Author:
  • Rajib Deb, P. P. Goswami
  • Total Page Count: 4
  • Page Number: 240 to 243

Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar -243 122, India

*This work is part of MVSc thesis submitted by the first author to IVRI, Izatnagar

Abstract

In the present study a gene encoding PPE protein of M.a.paratuberculosis (FJ032182) was cloned with murine IFN-, in a mammalian bicistronic vector pIRES 6.1 to elucidate the role of gamma interferon on the PPE gene for determination of CD4/CD8 ratio in post immunized mice. Flow cytometric analysis with mononuclear mice splenocytes on 42nd day post immunization revealed significant reduction of the CD4/CD8 ratio in the mice group immunized with pIR PPE/IFN (1.65±0.0001) compared to the pIR PPE group (2.92±0.0003). These result suggested that coexpression of murine IFNγ in conjunction with PPE protein significantly enhanced the CMI response.

Keywords

CD4/CD8, Gamma interferon, PPE, Mycobacterium avium paratuberculosis