1Division of Veterinary Biotechnology, Genetic Engineering of Bacteriology, Modular Lab Building., IVRI, Izatnagar.
2Indian Veterinary Research Institute, Izatnagar, Bareilly (Dist), Uttar Pradesh-243122, India.
Central Institute for Research on Buffaloes, Hisar-125 001, India
*Corresponding author: yosefdeneke@yahoo.com
Online published on 25 September, 2012.
Availability of developmentally competent buffalo oocytes is critical for in vitro embryo production and application of related biotecniques. The objective of the present study was to assess the effect of BSA in place of FCS as maturation media supplement on in vitro maturation buffalo oocytes. Oocytes were aspirated from abattoir ovarian follicles of 2-8 mm diameter followed by maturation in TCM-199 supplemented with hCG, PMSG and containing either 0.4% BSA (group-I) or 10% FCS (group-II). Based on cumulus expansion maturation rate was assessed among the two groups, group II showing a significant higher percentage values (89.1±3.5%) as compared to the group I (73.9±4.2%).
Buffalo oocytes, in vitro maturation, BSA, FCS
