Double molecular markers-assisted breeding of silkworm, Bombyx mori, resistant to the virus, BmNPV and BmDNV-Z

In order to improve the classic techniques of silkworm breeding and achieve the integration of good economic traits and disease resistance traits, a cross-breeding and molecular marker assisted breeding technology combined was developed in this experiment. The practical strain Jingsong and the strain NB, highly resistant to the BmNPV found from the library of silkworm races and strains in China, were selected as the breeding material. Through cross-breeding and backcrossing, the disease resistant gene was introducted into the economic strain Jingsong. At the same time, the strain Qiufeng, resistant to the BmDNV, was used as the donor material of resistance gene. In this experiment, a new strain resistant to the two virus, BmNPV and BmDNV-Z, was obtained through the method of virus administrion and molecular marker assisted breeding technology.