Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2010
  • Volume: 44
  • Issue: 2

Molecular Cloning of A Dna Fragment Encoding 34.9 KDA Carboxy Terminal Ppe Protein of Mycobacterium Avium Subsp. Paratuberculosis in E.Coli*

  • Author:
  • R. Deb, P.P. Goswami, V.K. Saxena, N.S. Prasad
  • Total Page Count: 4
  • Page Number: 131 to 134

Department of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, 243122, India.

*This work is the part of MVSc thesis work of the first author.

Abstract

Mycobacterium ar/ium ssp. Paratuberculosis (Map) is the causative agent of johne's disease in animals. A PPE (Proline Proline Glutamic acid rich) antigen encoding 34.9 kDa hypothetical protein of M avium subsp. ParatuberclIlosiswas cloned and sequenced. Sequence analysis revealed that no other published sequences having significant homology with that PPE gene.which elucidated that the PPE 34.9 kDa could be used as diagnostic antigen.

Keywords

PPE, 34.9 kDa, Mycobacterium avium ssp. Paratuberculosis, FJ032182