Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar-243 122, India.
The present study compared the efficacy of Microscopic Agglutination Test (MAT) with UpL32 and UpL41 recombinant antigen based Enzyme-linked immunosorbent assay (ELISA) for the diagnosis of bovine leptospirosis. The LipL32 and 41 genes were amplified using specific primers and the polymerase chain reaction products (PCR) were cloned. The gene inserts from positive recombinant clones were inserted into expression vector and transformed in Escherichia coli DH5á cells. The recombinant LipL32 and 41 proteins were expressed, purified and standardized. The optimum antigen concentration of 100ng of each antigen was used for the screening of serum samples from cattle suspected of leptospirosis. It was found that recombinant antigen ELISA detected more positive cases of illness than MAT. Between the two recombinant antigens ELISA, LipL32 ELISA was able to detect more positives.
Microscopic agglutination test (MAT), LipL32, LipL41, ELISA, Leptospirosis
