1College of Veterinary Science, Acharya N.G. Ranga Agricultural University, Rajendranagar, Hyderabad-500 030, India.
2BAIF, Urulikanchan, Pune.
3Department of Animal Science, College of Agriculture, Bijapur-586 101.
Lyophilized culture of Fusarium sporotrichioides was cultured on oatmeal agar. Subsequently the fungus was grown on broken wheat for three weeks for production of zearalenone toxin on large scale. Quantification of the cultured toxin was done using High Performance Thin Layer Chromatagraphy and found to be 573. 12 ppm. This culture was added to poultry feed to obtain the required level of toxin (2 ppm). This zearalenone contaminated feed was then treated with different adsorbents: yeast culture, activated charcoal, hydrated sodium calcium alumino silicate (HSCAS) and polyvinyl poly pyrrolidone (PVPP) at different levels to study the levels of adsorption of the toxin. The quantity of unbound zearalenone was estimated by ELISA method. Yeast culture and activated charcoal at 0.2% levels adsorbed toxin to a maximum extent (81.45 and 79.45%, respectively) and proved better than HSCAS and PVPP (40.23 and 66.90%, respectively).
