ICAR Scheme on DNA based diagnostics for Leptospirosis, Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore - 560 024, India.
Gene fragment of Leptospira serovar Canicola was cloned in E. coli cells using pUC18 plasmid as a vector. The recombinant cells were selected on LB agar medium, which contained ampicillin, X-gal and IPTG. From the recombinant white colonies, plasmid was extracted and the leptospiral DNA insert was collected after digestion with EcoR I and used as probe in hybridization experiments.
