Indian Journal of Animal Research
SCOPUSWeb of Science
  • Year: 2001
  • Volume: 35
  • Issue: 2

Optimization of polymerase chain reaction for detection of infectious bursal disease virus

  • Author:
  • M. Parthiban, T.D. Sujatha, V. Thiagarajan, R. Velmurugan
  • Total Page Count: 3
  • Page Number: 132 to 134

Tamil Nadu Veterinary and Animal Sciences University, Chennai - 600 051, India.

*Present address: Dept. of Animal Biotechnology, Madras Veterinary College, Chennai - 600 057, India.

Abstract

Optimisation of reagents and conditions used in PCR has been carried out for detection of infectious bursal disease virus. Varying concentration of Taq DNA polymerase and dNTP mix does not much affect the PCR assay whereas varying concentration of MgCl2, IBD specific primers and annealing temperature has pronounced effect on the amplification reaction.