College of Veterinary Science, Birsa Agricultural University, Ranchi, Bihar-834 007, India.
1Present address: Institute of Animal Health-cum-Biological Products, Zakura, Srinagar-190 006, India.
Oocytes Liberated from abattoir goat ovaries were cultured in tissue culture medium-199, supplemented with heat inactivated oestrus cow serum, at 39°C for 24 hours. A total of 225 cultured oocytes were studied for nuclear maturation with 40.44% oocytes maturing to metaphase-II, 28.81% in different stages of maturation (mataphase-I to telophase-I), 14.66 in germinal vesicle stage and 16% oocytes were observed abnormal.