Genetic variation of acid lime genotypes as assessed by microsatellite markers

Evaluation of genetic dissimilarity is imperative to the plant breeder for improvement of high yielding varieties and hybrids. A large variability present in acid lime crop due to cross pollinated crop. In the present study, an effort was made to assess genetic diversity and their genetic relatedness of 14 acid lime genotypes collected from diferent locations and maintained at All India Coordinated Research Project on Fruits, Dr.P.D.K.V., Akola by using Simple Sequence Repeate markers.The average genetic similarity level among the 14 acid lime genotypes was 0.97 and ranged from 0.17 to 0.97. The cluster analysis separated the genotypes into three major groups.Total 52 allele were detected by ten primer pairs and size of allele ranged from 100 to 1000 bp. Average polymorphic information content (PIC) value was 0.72 whereas, the highest 0.86 and the lowest 0.38 was observed in AG-14 and CAT-01 markers, respectively.The results clearly showed that, the high level of genetic diversity was observed among the genotypes and SSR markers tested in the present study was highly polymorphic and more informative for the assessment of genetic diversity of acid lime genotypes.