1PhD Scholar, Department of Plant Breeding and Genetics, Agricultural College & Research Institute, Madurai-625104, India
2Professor and Head, Department of Plant Breeding and Genetics, Agricultural College & Research Institute, Madurai-625104, India
3Professor & Head, Department of Millets, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India
4Professor and Head, Department of Plant Pathology
5Professor, Department of Soil science & Agricultural Chemistry
*E-Mail: mrameshagri@gmail.com
Online published on 2 July, 2019.
An experiment was conducted to estimate the lethal dose of the chemical mutagen EMS and physical mutagen gamma ray in barnyard millet variety Co(Kv) 2. Genetically pure seeds were treated with different doses of EMS viz. 30mM, 40mM, 50mM, 60mM, 70mM, 80mM, 90mM and 100mM and gamma rays viz., 500 Gy, 600 Gy, 700 Gy, 800 Gy, 900Gy and 1000 Gy. The LD50 values were observed based on growth reduction of seedlings after EMS and gamma ray treatment. The LD50 dose for EMS and gamma ray under in vitro and in vivo condition were fixed at 70mM and 800 Gy respectively, based on probit analysis. As the doses of applied EMS and gamma ray increased, there was a decrease in germination, survival rate of seedlings, root length, shoot length, seedling height, vigour index under in vitro conditions and emergence and survival under field (in vivo) conditions in M1 generation as compared to the control.
