Asian Journal of Pharmacy and Technology
  • Year: 2018
  • Volume: 8
  • Issue: 2

Application of UV Spectrophotometric Methods for Simultaneous Estimation of Emtricitabine and Tenofovir Alafenamide Fumarate in Bulk

  • Author:
  • Ashwini Shelke, Madhavi Shinde1, Rajendra Mogal2, Rahul Sable3, Anil Jadhav4
  • Total Page Count: 5
  • Page Number: 103 to 107

1Department of Pharmaceutical Chemistry, Sandip Institute of Pharmaceutical Sciences, Nashik, India-422213

2Department of Pharmaceutics, Sandip Institute of Pharmaceutical Sciences, Nashik, India-422213

3Department of Pharmacology, Sandip Institute of Pharmaceutical Sciences, Nashik, India-422213

4Department of Pharmacognosy, Sandip Institute of Pharmaceutical Sciences, Nashik, India-422213

*Corresponding Author E-mail: shelke.ashwini2@gmail.com

Online published on 21 July, 2018.

Abstract

Two simple method was developed for the simultaneous determination of tenofovir alafenamide fumarate (TAF) and emtricitabine (EMT) in combined tablets. The method involves the Simultameous equation method (Method A) and Q-Absorabnce Method (Method B). Tenofovir (TEF) has λmax 260nm and Emtricitabine (EMT) shows λmax at 237nm and 280nm both. Tenofovir and 280nm shows linearity in a concentration range of 5–30 μg/mL and employs formation and solving of simultaneous equation using wavelengths for both drugs using Distilled water. Method B employs measurement using 237nm (λmax of EMI) and 251nm as Iso-absorptive Point.

Keywords

Tenofovir Alafenamide fumarate, Emtricitabine, Multicomponent analysis, ICH guideline, Validation